| 產(chǎn)品編號 | bsm-60637R |
| 英文名稱 | Rabbit Anti-Claudin 18.2 antibody |
| 中文名稱 | 緊密連接蛋白18重組兔單抗 |
| 別 名 | Claudin-18; CLD18_HUMAN; CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 轉(zhuǎn)運蛋白 細胞粘附分子 內(nèi)皮細胞 跨膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | R7G12 |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Claudin 18 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Claudin superfamily consists of many structurally related proteins in humans (1). These proteins are important structural and functional components of tight junctions in paracellular transport (1,2). Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues (1). Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and Junction adhesion molecule (3). Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands (4). Emerging evidence suggests that the Claudin family of proteins regulates transport through tight junctions via differential discrimination for solute size and charge (5).Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions (6). SWISS: P56856 Gene ID: 51208 Database links: Entrez Gene: 51208 Human Entrez Gene: 56492 Mouse Omim: 609210 Human SwissProt: P56856 Human SwissProt: P56857 Mouse Unigene: 655324 Human Unigene: 386784 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium EDTA buffer (9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 18.2) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 18.2) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium EDTA buffer (9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 18.2) Monoclonal Antibody, Unconjugated (bsm-60637R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
