| 產品編號 | bs-3996R |
| 英文名稱 | Rabbit Anti-MDH1 antibody |
| 中文名稱 | 可溶性蘋果酸脫氫酶抗體 |
| 別 名 | Cytosolic malate dehydrogenase; Malate dehydrogenase 1, NAD (soluble); MDHA; MDHs; Soluble malate dehydrogenase; Malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1; Malate dehydrogenase 1 NAD (soluble); Malate dehydrogenase 2; Malate dehydrogenase cytoplasmic; Malate dehydrogenase 2 NAD (mitochondrial); MDH 1; MDH 2; MDH; MDH2; MDHA; MDHm; MDHs; MMDH; Mor 1; Mor 2; Mor1; MOR2; cytoplasmic; MDH s; MDHC_HUMAN; MGC:1375; SMDH; Soluble malate dehydrogenase. |
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Specific References (2) | bs-3996R has been referenced in 2 publications.
[IF=19.328] Patricia Altea-Manzano. et al. Reversal of mitochondrial malate dehydrogenase 2 enables anaplerosis via redox rescue in respiration-deficient cells. MOL CELL. 2022 Nov;: WB ; Human.
[IF=6.1] Paul, Subhojit, et al. "STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO 2 gradient." Redox Biology (2017). WB ; Rat.
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| 研究領域 | 腫瘤 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=0.2ug/Test,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MDH1: 265-334/334 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. Malate dehydrogenase 1 (MDH1) is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Subunit: Homodimer. Subcellular Location: Cytoplasm. Post-translational modifications: ISGylated. Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation. Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family. SWISS: P40925 Gene ID: 4190 Database links: Entrez Gene: 4190 Human Entrez Gene: 17449 Mouse Omim: 154200 Human SwissProt: P40925 Human SwissProt: P14152 Mouse Unigene: 526521 Human Unigene: 212703 Mouse Unigene: 13492 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Stomach tissue lysates
Lane 5: Rat Heart tissue lysates
Lane 6: Rat Cerebrum tissue lysates
Lane 7: Rat Stomach tissue lysates
Lane 8: Human HL-60 cell lysates
Lane 9: Human MOLT4 cell lysates
Primary: Anti-MDH1 (bs-3996R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MDH1) Polyclonal Antibody, Unconjugated (bs-3996R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-MDH1 antibody (bs-3996)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-DMT1 antibody (bs-3996R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
